The applied biosystems 7900ht fast realtime pcr system employs the automation controller software for automated operation of the 7900ht instrument. Real time pcr model applied biosystems 7500 now on sale at the lab world group. Applied biosystems 730075007500 fast real time pcr system and the applied biosystems 730075007500 fast sds software. Applied biosystems 7500 fast realtime pcr system, desktop. The applied biosystems 7900ht fast realtime pcr system is covered by u. Apr 29, 2014 applied biosystems 7500 real time pcr system.
Fast pcr option reduces run time to about 35 minutes in a standard 96well. Applied biosystems 7900ht user manual 384 pages fast real time pcr system and sds enterprise database brand. Vantage offers real time pcr services to assist users in verifying microarray results. After gathering some information about what the market of real time pcr machines offers, were not really sure which. Applied biosystems 7900ht fast real time pcr for sale online. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. The running royalty component of that license may be purchased from applied biosystems or obtained by purchasing authorized reagents. Abi prism 7900ht sequence detection system users manual. Applied biosystems 7500 fast and 7500 realtime pcr system. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr researchers.
Select the ramp rate tab, then verify the default settings. Applied biosystems 7900ht fast realtime pcr system from. Perform the manual method for converting total rna to cdna. Abi 7500 fast real time pcr system with 96 well block. Applied biosystems 7500 fast and 7500 realtime pcr. The 7900ht fast real time pcr system is the only real time quantitative pcr system that combines 384well plate compatibility with fully automated robotic loadingand now also offers optional fast real time pcr capability. For use with equipment 310 genetic analyzer, 3xl genetic analyzer, 3500xl dx genetic analyzer, 3730xl dna analyzer, 7500 fast system, 7900ht fast system, steponeplus, veriti dx fast thermal cycler, veriti fast thermal cycler, 3730 dna analyzer, 3 genetic analyzer, 3500 dx genetic analyzer, 7500 fast dx system, viia 7 dx fast system. This flexible system offers multiple block configurations, software choices, and an optional automation. The technical manual listed below, complete protocol, describes the plexor hy system and provides instructions for use with the applied biosystems 7500 and 7500 fast realtime pcr systems, including reaction setup, realtime pcr instrument programming and data analysis using the plexor analysis software. The abi 7500 fast real time pcr is a must for any genetics research lab looking to save on space while outfitting their lab with a realiable real time pcr system. Applied biosystems quantstudio 6 flex realtime pcr system, 96well fast, laptop facilitates real time pcr based applications with an upgradable block selection, allowing you to plan for experiments today and grow with tomorrows needs.
Applied biosystems 7300 real time pcr system the abi 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. Applied biosystems 7500 realtime pcr system, desktop. Applied biosystems 7900ht fast real time pcr system plusminus assay guide. Applied biosystems 7900ht manuals manuals and user guides for applied biosystems 7900ht. Applied biosystems 7500 getting started pdf download. For our lab we are considering the purchase of a new real time pcr machine. User bulletin applied biosystems 7900ht fast real time pcr system 112008 subject. Notice to purchaser the applied biosystems 7900ht fast realtime pcr system is a realtime thermal cycler covered by us patents and corresponding claims in their nonus counterparts, owned by applied biosystems.
Sequence detection systems software or manually set and. Applied biosystem abi prism 7900ht sequence detection system rt pcr lot 460. The automation accessory combined with 384well plate and 7900ht micro fluidic card capability make the 7900ht system. Abi prism 7900ht sequence detection system user guide. Sequence detection systems software or manually set and that is used for. The applied biosystems 7900ht fast real time pcr system employs the automation controller software for automated operation of the 7900ht instrument.
Abi 7500 fast realtime pcr system the lab world group. The applied biosystems 7900ht fast real time pcr system is a sequence detection system instrument designed for automated, highthroughput detection of fluorescent pcr related chemistries. Applied biosystems abi 7500 real time pcr video duration. The hrm software is available on the 7500 fast real time pcr system, which delivers precise results in a standard 96well format. New real time pcr user instructions university of illinois at. Other machines similar to applied biosystems 7500, fast real time pcr. Applied biosystems abi prism 7900ht realtime qpcr system. The applied biosystems 7900ht fast realtime pcr system is a realtime thermal. Applied biosystems abi prism 7900ht sequence detection system unit power on, has not been tested further. Applied biosystems microamp enduraplate optical 96well fast clear reaction plates with barcode 20 plates life sciences. The quantstudio 5 realtime pcr system is compact in form, factory calibrated and is delivered in userfriendly packaging to enable fast and simple installation for immediate use.
Abi prsi m 70007700 7900ht sequence detection systems and the applied biosystems 7300 real time pcr system, the use of rox reference dye lsr 50x is recommended. Performing maintenance and thermo fisher scientific. Intended for laboratory staff responsible for the installation and maintenance of the stepone or steponeplus system 4376782 applied biosystems stepone and steponeplus realtime pcr. Installing the fast 96well block and fast 96well plate adapter. Applied biosystems microamp optical 384well reaction plate with barcode 50 plates life. The great thing about the abi 7300 realtime pcr is theres no need to purify pcr, run any gels, or implement post pcr alterations. Applied biosystems amplitaq gold fast pcr master mix 25. Plexor hy system for the applied biosystems 7500 and 7500. The applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide describes how to prepare, maintain, and troubleshoot the applied biosystems 7900ht fast realtime pcr system instrument, zymark twister microplate handler, and sds enterprise database. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. Openarray plate is compatible with the quantstudio 12k flex real time pcr system. Relative quantitation using comparative c getting started.
Complete the plate document setup as described in the applied biosystems 7900ht fast real time pcr system and sds enterprise database user guide. The applied biosystems 7300 real time pcr runs on its own software platform and quantitative results are available immediately, once pcr run is complete. Applied biosystems 7500 fast realtime pcr systems spectral. The applied biosystems 7900ht fast realtime pcr system is a realtime thermal cycler covered by us patents and corresponding claims in their nonus counterparts, owned by applied biosystems. Veriti fast thermal cycler, viia 7 dx fast system, viia 7 fast system. Manual ct the sds software calculates baseline and threshold values for a. Keck center 7900 ht realtime quantitative pcr practical. Fast pcr, pcr, pcr and real time pcr, sanger sequencing, sanger sequencing technology and. Below are some the key features and benefits of the abi 7900ht. The abi 7500 is the predecessor of the abi 5700, made as the lower throughput version of the abi 7900ht. User interchangeable block formats deliver multiple throughput options in a system that can expand and adapt to meet future requirements. The overall size and footprint of the abi 7500 was made more compact. In 20012010, rtpcr has been used as a powerful tool for genotyping applied biosystems abi7900 fast realtime pcr system user guide, biosystems 730075007500, alker, 2004, quantifying viral load, and gene copy number assays. Expect that the laser in this unit needs to be replaced i.
Applied biosystems 7500 real time pcr system youtube. Five user attention words appear in the text of all applied biosystems user documentation. Applied biosystems 75007500 fast real time pcr system getting started guide for genotyping experiments v preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems 75007500 fast real time pcr system 75007500 fast system. Taqman array micro fluidic cards user guide pn 4400263c. Searching for a new real time pcr machine which one. The use of rox reference dye lsr or lmp is optional.
Applied biosystems abi prism 7900ht sequence detection system. The applied biosystems 7900ht fast realtime pcr system is a fully automated, high throughput system that allows users to run realtime. Applied biosystems 7500 fast dx real time pcr system patrick luetdke, director of public health at the unified state laboratories in taylorsville, utah discusses how the applied biosystems 7500. Applied biosystems 7900ht fast realtime pcr system user. Asis the abi prism 7900ht sequence detection system is a highthroughput real time pcr system that detects and quantitates nucleic acid sequences. Applied biosystems 7500 fast real time pcr system for sale. For the applied biosystems 7500 real time pcr system and the stratagene mx3000p, the use of rox reference dye lmp is recommended. The following user attention words appear in applied biosystems user documentation. Label license the 75007500 fast real time pcr system is covered by us p atents, and corresponding claims in their nonus counterparts, owned by a pplied biosys tems. User bulletin applied biosystems realtime pcr systems july 2007 subject. Userinterchangeable thermal cycling block formats let.
This is the users manual for the abi prism 7900ht sequence detection system. The applied biosystems proflex pcr system combines. See user s manual or package insert for limited label license, and trademark information. Complete the plate document setup as described in the applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide.
Find and purchase 7900ht fast real time pcr system products at invitrogen life science technologies. Applied biosystems abi prism 7900ht realtime pcr system. Abi 7500 fast real time pcr system with 96 well block available from the lab world group. The 7500 real time pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility.
Portable document format pdf versions of this guide and the applied biosystems 730075007500 fast real time pcr system installation guide, online help, and getting started guides are also available on the applied biosystems. The 7900ht fast realtime pcr system is a realtime quantitative pcr system that combines 96 and 384well plate compatibility and the taqman low density array with fully automated robotic loadingand now also offers optional fast realtime pcr capability. Applied biosystems 7900ht fast real time pcr system 6 user bulletin d. The quantstudio 5 real time pcr system is compact in form, factory calibrated and is delivered in user friendly packaging to enable fast and simple installation for immediate use. Pcr process for internal research and development using this instrument. About the comparative c t method for relative quantitation the taqman array is designed for twostep rtpcr. Applied biosystems 7900ht fast realtime pcr system 96. Real time pcr system supported software versions file extension applied biosystems 7900ht fast real.
May 10, 2005 the applied biosystems 7500 realtime pcr system is a 3rd generation of thermocycling technology that allows quanitation of product generation in real time. Applied biosystems 7900ht fast realtime pcr system user bulletin. Applied biosystems 7900ht fast realtime pcr system and sds. Applied biosystems 7900ht fast realtime pcr system. Apr 27, 2011 applied biosystems 7500 fast dx real time pcr system patrick luetdke, director of public health at the unified state laboratories in taylorsville, utah discusses how the applied biosystems 7500. Realtime pcr systems chemistry guide pn 4378658 rev a. Applied biosystems abi prism 7900ht sequence detection. Dyes which can be used on the applied biosystems 7500 fast include cy3, cy5, fam, joe, ned, rox, sybr, green, tamra, texas red,and vic. Relative quantitation rq algorithms in applied biosystems realtime pcr systems software. Openarray plate is compatible with the quantstudio 12k flex realtime pcr system. This guide describes the procedure for conducting plusminus assays using the 7900ht fast real time pcr system.
The applied biosystems 7900ht fast realtime pcr system is a realtime thermal cycler. This flexible system offers multiple block configurations, software choices, and an optional automation accessory, all designed to deliver the throughput and features you need. Laboratory equipment applied biosystems 7900ht user manual. Fast real time pcr system and sds enterprise database 384 pages. Applied biosystems qpcr analysis modules within the thermo fisher cloud are web browserbased software tools that are flexible, fast and easy to use.
Abi 7900ht thermo scientific 4329001 fast realtime pcr. Applied biosystems 7900ht fast real time pcr system and sds enterprise database user guide pn 4351684 applied biosystems 7900ht fast real time pcr system user bulletin. View online or download applied biosystems 7500 fast installation and maintenance manual, getting started. Applied biosystems 7900ht fast realtime pcr system relative. The following user attention words appear in applied biosystems user. Applied biosystems 7900ht fast realtime pcr system maintenance and troubleshooting guide pn 4365542 applied biosystems 7900ht fast realtime pcr system site preparation guide pn 4351923 abi prism 6100 nucleic acid prepstation users guide pn 4326242 dna isolation from fresh and frozen blood, tissue culture cells, and buccal. Abi 7900ht thermo scientific 4329001 fast real time pcr 4330966 w. Applied biosystems quantstudio 5 realtime pcr system, 96. Select the data collection tab, then verify the default settings. We also offer the 7500 fast dx instrument for your diagnostic needs.
The abi 7300 is great quantifying nucleic acid sequences without compromising data. We also provides full service genotyping on this platform. User bulletin applied biosystems 7900ht fast realtime pcr system 112008 subject. When you install the fast 96well block, make sure that you also install the fast 96well plate adapter. The purchase price of this base unit includes the upfront fee component of a. Vantage offers real time services on the openarray and taqman platforms. This small but robust pcr takes up less space than the applied biosystems 7900ht. Home repair real time pcr systems abi 7900ht7900htfast real time pcr system abi 7900ht7900htfast real time pcr system a real asset to any disease research program, the abi 7900ht is the only real time quantitative pcr system that combines 384well plate compatibility with fully automated robotic loading. Applied biosystems quantstudio 6 flex realtime pcr system.
Applied biosystems microamp optical 384well reaction. It supports many real time pcr applications for custom assays like gene expression, snp genotyping, viral load analysis, mirna qualification, and pathogen. Thermo fisher scientific 7900ht fast realtime pcr system. The cores instruments are available either for self service or full service. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling real time pcr melt curve assays to be used more accurately for mutation scanning and genotyping. Make sure that the plate does not have any adhesive, or film protruding beyond the perforation. Applied biosystems 7900ht user manual pdf download. The applied biosystems 7900ht fast real time pcr system is a high throughput real time pcr system that detects and quantitates nucleic acid sequences.
Perform the manual method for converting total rna. Applied biosystems 7500 fast realtime pcr systems spectral calibration kit ii easytouse, preloaded, optical reaction plates enable quick and easy calibration of the 7500 fast realtime pcr system. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. Applied biosystems 7900ht fast realtime pcr system allelic discrimination getting started guide pn 4364015 applied biosystems 7900ht fast realtime pcr system absolute quantitation using standard curve getting started guide pn 4364014 applied biosystems 7900ht fast realtime pcr system plusminus getting started guide pn 4364017. In the reversetranscription rt step, cdna is reverse transcribed from total rna samples using. Configuring the the software coordinates the action of the 7900ht instrument, the bar code reader, software and the plate handler while acquiring and saving raw data during each run. I would suggest the step one or step one plus systems from applied biosystems. Applied biosystems 75007500 fast thermo fisher scientific. The robot cannot position sticky plates correctly inside of the instrument.
No right is conveyed expressly, by implication or by estoppel under any other patent claim, such as claims to. Applied biosystems 7900ht fast realtime pcr system and. Rtpcr has been the gold standard of gene expression level assay. The workspace provides quick access to all elements of the software. Steponeplus realtime pcr systems installation, networking, and maintenance guide explains how to install and maintain the stepone and steponeplus systems. Applied biosystems 7500 realtime pcr system biocompare. Applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide iii. View online or download applied biosystems 7900ht user manual. Applied biosystems 7500 realtime pcr systems offer you high performance, multicolor realtime pcr wherever your research takes you. Performing fast gene quantification pn 4352533 rq manager software user guide pn 4351670 snp manager software user guide pn 4351671. The 7900ht fast realtime pcr system is the only realtime quantitative pcr system that combines 96 and 384well plate compatibility and the taqman low density array with fully automated robotic loadingand now also offers optional fast realtime pcr capability. Applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide pn 4351684, with any changes as noted below. Applied biosystems 7500 fast dx realtime pcr system.
Applied biosystems 7900ht fast realtime pcr system 6 user bulletin d. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. An optional upgrade to the 7500 fast system is available. Taqman array micro fluidic cards user guide v preface how to use this guide purpose of this guide the applied biosystems taqman array micro fluidic cards user guide provides information for ordering taqman array micro fluidic cards, preparing and running the taqman array cards on an applied biosystems 7900ht fast realtime pcr system. Designed to work with applied biosystems 384well real time pcr systems and thermal cyclers. Applied biosystems 7300 real time pcr system andbio. This 7500 fast system uses our master mix formulations and enables you to shorten your real time pcr runs to as little as 30 minutes. Applied biosystems microamp enduraplate optical 96well. The plexor hy systemaf is a novel realtime pcr system for quantification of total human autosomal and human male y dna within a sample.
Flexibility the 7900ht system is a versatile research tool that can accommodate any realtime pcr need. This technical manual describes the plexor hy system and provides instructions for use with the applied biosystems 7500 and 7500 fast realtime pcr systems, including reaction setup, realtime. Used applied biosystems abi 7900ht fast pcr the 7900ht is said to be a more sensitive device than the newer models that use led light sources, but its laser that needs to be replace every so often. Applied biosystems microamp fast optical 96well reaction.
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